Looking under the hood of cells: from single molecule dynamics to whole cell organelle reconstructions

Pathology Grand Rounds

Friday, September 30, 2022 at 1:00 PM


Grand Round starts in:

MART Auditorium - Stony Brook , NY
September 30th, 2022
1.00 AMA PRA Category 1 Credit Hours

Description

Looking under the hood of cells: from single molecule dynamics to whole cell organelle reconstructions



Jennifer Lippincott-Schwartz, Janelia Research Campus, HHMI, Ashburn, VA



Powerful new ways to image the internal structures and complex dynamics of cells are revolutionizing cell biology and bio-medical research. In this talk, I will focus on how emerging fluorescent technologies are increasing spatio-temporal resolution dramatically, permitting simultaneous multispectral imaging of multiple cellular components. In addition, results will be discussed from whole cell milling using Focused Ion Beam Electron Microscopy (FIB-SEM), which reconstructs the entire cell volume at 4 voxel resolution. Using these tools, it is now possible to begin constructing an “organelle interactome”, describing the interrelationships of different cellular organelles as they carry out critical functions. I will also present an additional tool, single particle tracking, describing how it can be used to characterize the dynamics of organelle contact sites and the behavior of tethering proteins. Together, the new tools are revealing new properties of organelles and their trafficking pathways, and how disruptions of their normal functions due to genetic mutations may contribute to important diseases.

Dates and Times

Start: 9/30/2022 1:00 PM
End: 9/30/2022 2:00 PM

Objectives

Objectives:



Describe new tools for imaging cells at nanometric resolution

Describe new results examining organelle-organelle contact sites

Provide an exciting framework to encourage students to study biological questions using imaging approaches in intact cells.

Speakers

  • Dr. Jennifer Lippincott-Schwartz

Location

MART Auditorium
101 Nicolls Road
Stony Brook , NY 11780

Accreditation

The School of Medicine, State University of New York at Stony Brook, is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians.

The School of Medicine, State University of New York at Stony Brook designates this live activity for a maximum of 1.00 AMA PRA Category 1 Credit(s) . Physicians should only claim the credit commensurate with the extent of their participation in the activity.

Need help with this Grand Round Session?

Please contact the Grand Round coordinator listed below:

Jessica Mattera
Department: Pathology Department
Phone: (631) 444-2169
Email: jessica.mattera2@stonybrookmedicine.edu

Give us a call
(631) 444-2094
Office of Continuing Medical Education
Renaissance School of Medicine at Stony Brook University
HSC, Level 2, Room 142
Stony Brook, New York 11794-8222